Review



anti sars cov 2 n  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    Sino Biological anti sars cov 2 n
    Anti Sars Cov 2 N, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/SARS-CoV+%2F+SARS-CoV-2+Nucleocapsid+Antibody%2C+Rabbit+MAb/pm42037405-345-5-8
    Average 97 stars, based on 379 article reviews
    anti sars cov 2 n - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Staining:

    Article Title: A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories
    Article Snippet: .. Plates were washed in PBS and stained with rabbit anti-nucleocapsid (Sino Biological, Eschborn, Germany; 1:2000) in PBS containing 0.1% bovine serum albumin (BSA). .. Cells were washed in PBS and stained with goat anti-rabbit Alexa Fluor 488 (Invitrogen, Waltham, MA, USA; 1:4000) in PBS containing 0.1% BSA.

    Article Title: SARS-CoV-2 Omicron entry is type II transmembrane serine protease-mediated in human airway and intestinal organoid models
    Article Snippet: .. Cells were blocked in 0.6% BSA (bovine serum albumin; Sigma) in PBS and stained with rabbit anti-nucleocapsid (Sino biological; 1:2,000) in PBS containing 0.6% BSA, washed thrice in PBS, and stained with goat anti-rabbit Alexa Fluor 488 (Invitrogen; 1:4,000) in PBS containing 0.6% BSA. .. Cells were washed twice in PBS, and plates were scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 μm; GE Healthcare).

    Article Title: Syrian hamster convalescence from prototype SARS-CoV-2 confers measurable protection against the attenuated disease caused by the Omicron variant.
    Article Snippet: .. Foci were stained with rabbit anti-nucleocapsid (Sino Biological, 40588-T62) diluted 1:1000 in 0.2% (w/v) Triton X-100/PBS for 1 h at room temperature. .. Anti-rabbit IgG HRP (Invitrogen, G-21234) was diluted 1:4000 in 0.2% (w/ v) Triton X-100/PBS and incubated with cells for 1h at room temperature.

    Article Title: A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories.
    Article Snippet: .. Plates were washed in PBS and stained with rabbit anti-nucleocapsid (Sino Biological, Eschborn, Germany; 1:2000) in PBS containing 0.1% bovine serum albumin (BSA). .. Cells were washed in PBS and stained with goat anti-rabbit Alexa Fluor 488 (Invitrogen, Waltham, MA, USA; 1:4000) in PBS containing 0.1% BSA.

    Article Title: SARS-CoV-2 Omicron entry is type II transmembrane serine protease-mediated in human airway and intestinal organoid models.
    Article Snippet: .. Cells were blocked in 0.6% BSA (bovine serum albumin; Sigma) in PBS and stained with rabbit anti-nucleocapsid (Sino biological; 1:2,000) in PBS containing 0.6% BSA, washed thrice in PBS, and stained with goat anti-rabbit Alexa Fluor 488 (Invitrogen; 1:4,000) in PBS containing 0.6% BSA. .. Cells were washed twice in PBS, and plates were scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 μm; GE Healthcare).

    Article Title: Pulmonary lesions following inoculation with the SARS-CoV-2 Omicron BA.1 (B.1.1.529) variant in Syrian golden hamsters
    Article Snippet: .. Cells were blocked in 3% BSA (bovine serum albumin; Sigma) in PBS and stained with rabbit anti-nucleocapsid (Sino biological; 1:2000) in PBS containing 0.1% BSA, washed thrice, and stained with donkey anti-rabbit Alexa Fluor 488 (Invitrogen; 1:4000) in PBS containing 0.1% BSA. .. Plates were washed and scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 25 μm; GE Healthcare).

    Membrane:

    Article Title: A stable subgenomic reporter coronavirus enables transcriptional profiling of bystander cells
    Article Snippet: Cell lysates (5-10 ug total protein) were separated by SDS-PAGE using Any kD precast gels (Bio-Rad) and transferred to 0.45-um nitrocellulose membrane by semidry electrotransfer. .. Membranes were probed with sheep polyclonal anti-Nucleocapsid anti-serum (MRC PPU & CVR Coronavirus Toolkit, Sheep No. DA116, 1:1000) , rabbit anti-Nucleocapsid (40643-T62, Sino Biological, 1:1000), anti-Spike (PAB21478-100, The Native Antigen Company, 1:1000), anti-SARS-CoV Membrane (ABIN1887462, Antibodies Online, 1:200), anti-MHV nucleocapsid J3.3 ( ) (1:200), anti-GAPDH (AM4300, Invitrogen, 1:4000) and anti-HSP90 (MA5-35624, Invitrogen, 1:2000). .. IR-dye labelled secondary antibodies (Li-Cor) were visualised using an Odyssey CLx imaging system (Li-Cor).



    Similar Products

    anti sars cov 2 n  (Sino Biological)
    97
    Sino Biological anti sars cov 2 n
    Anti Sars Cov 2 N, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/SARS-CoV+%2F+SARS-CoV-2+Nucleocapsid+Antibody%2C+Rabbit+MAb/pm42037405-345-5-8
    Average 97 stars, based on 1 article reviews
    anti sars cov 2 n - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    horseradish peroxidase hrp antibody  (Sino Biological)
    94
    Sino Biological horseradish peroxidase hrp antibody
    Horseradish Peroxidase Hrp Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/SARS-CoV+%2F+SARS-CoV-2+Nucleocapsid+Antibody+(HRP)%2C+Rabbit+MAb/pm42037405-289-5-13
    Average 94 stars, based on 1 article reviews
    horseradish peroxidase hrp antibody - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    rabbit anti hcov nl63 n antibody  (Sino Biological)
    94
    Sino Biological rabbit anti hcov nl63 n antibody
    Rabbit Anti Hcov Nl63 N Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/Human+coronavirus+(HCoV-NL63)+Nucleocapsid+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/pm42037405-351-20-27
    Average 94 stars, based on 1 article reviews
    rabbit anti hcov nl63 n antibody - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    anti hcov oc43 n rabbit polyclonal antibody  (Sino Biological)
    95
    Sino Biological anti hcov oc43 n rabbit polyclonal antibody
    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of <t>HCoV-OC43</t> N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
    Anti Hcov Oc43 N Rabbit Polyclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/Human+coronavirus+(HCoV-OC43)+Nucleocapsid+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/bio_rxiv__64898__2026__04__22__720112-153-23-30
    Average 95 stars, based on 1 article reviews
    anti hcov oc43 n rabbit polyclonal antibody - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    anti sars cov 2 nucleocapsid protein  (Sino Biological)
    97
    Sino Biological anti sars cov 2 nucleocapsid protein
    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of <t>HCoV-OC43</t> N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
    Anti Sars Cov 2 Nucleocapsid Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/SARS-CoV+%2F+SARS-CoV-2+Nucleocapsid+Antibody%2C+Rabbit+MAb/pm41963730-265-40-43
    Average 97 stars, based on 1 article reviews
    anti sars cov 2 nucleocapsid protein - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    primary antibodies against sars cov 2 nucleocapsid protein  (Sino Biological)
    97
    Sino Biological primary antibodies against sars cov 2 nucleocapsid protein
    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of <t>HCoV-OC43</t> N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
    Primary Antibodies Against Sars Cov 2 Nucleocapsid Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/SARS-CoV+%2F+SARS-CoV-2+Nucleocapsid+Antibody%2C+Rabbit+MAb/pm41963730-245-4-10
    Average 97 stars, based on 1 article reviews
    primary antibodies against sars cov 2 nucleocapsid protein - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    monoclonal rabbit anti sars cov 2 nucleocapsid antibody  (Sino Biological)
    96
    Sino Biological monoclonal rabbit anti sars cov 2 nucleocapsid antibody
    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of <t>HCoV-OC43</t> N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
    Monoclonal Rabbit Anti Sars Cov 2 Nucleocapsid Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/SARS-CoV+%2F+SARS-CoV-2+Nucleocapsid+Antibody%2C+Rabbit+MAb/pm41957014-165-5-10
    Average 96 stars, based on 1 article reviews
    monoclonal rabbit anti sars cov 2 nucleocapsid antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    p re ss anti sars cov 2 nucleocapsid protein antibody  (Sino Biological)
    97
    Sino Biological p re ss anti sars cov 2 nucleocapsid protein antibody
    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of <t>HCoV-OC43</t> N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
    P Re Ss Anti Sars Cov 2 Nucleocapsid Protein Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/SARS-CoV+SARS-CoV-2+Nucleocapsid+Antibody%2C+Rabbit+MAb/pm41935038-478-32-39
    Average 97 stars, based on 1 article reviews
    p re ss anti sars cov 2 nucleocapsid protein antibody - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    anti sars cov sars cov 2 nucleocapsid  (Sino Biological)
    97
    Sino Biological anti sars cov sars cov 2 nucleocapsid
    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of <t>HCoV-OC43</t> N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
    Anti Sars Cov Sars Cov 2 Nucleocapsid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/SARS-CoV+%2F+SARS-CoV-2+Nucleocapsid+Antibody%2C+Rabbit+MAb/pmc13098204-244-32-34
    Average 97 stars, based on 1 article reviews
    anti sars cov sars cov 2 nucleocapsid - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    rabbit  (Novus Biologicals)
    94
    Novus Biologicals rabbit
    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of <t>HCoV-OC43</t> N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
    Rabbit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nucleocapsid/SARS-CoV-2+Nucleocapsid+Antibody+(ARC2372)+-+BSA+Free/pmc13043052-98-3-5
    Average 94 stars, based on 1 article reviews
    rabbit - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of HCoV-OC43 N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

    Journal: bioRxiv

    Article Title: Proximity proteomics reveals a role for IFI16 during human coronavirus infection

    doi: 10.64898/2026.04.22.720112

    Figure Lengend Snippet: IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of HCoV-OC43 N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

    Article Snippet: Cells were washed twice with a permeabilization/wash buffer (0.1% saponin and 0.5% BSA in 1X PBS) and stained with a 1:10000 dilution of anti-HCoV-OC43-N rabbit polyclonal antibody (catalogue no. 40643-T62; Sino Biological) for 30 min at 4°C.

    Techniques: Infection, CRISPR, Knock-Out, Single Cell, Clone Assay, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Incubation, Staining, Flow Cytometry